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Challenge HF® Hollow Fiber Cartridges

Challenge HF® Hollow Fiber Cartridges
Challenge Filair® PTFE High-Retention, High-Flux Hydrophobic Sterilizing-Grade Capsule Filters & Cartridges

Challenge Filair® PTFE High-Retention, High-Flux Hydrophobic Sterilizing-Grade Capsule Filters & Cartridges
Challenge Pore® PES High Mycoplasma Retention Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES High Mycoplasma Retention Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PES Low Protein Binding Hydrophilic Sterilizing-Grade

Challenge Pore® PES Low Protein Binding Hydrophilic Sterilizing-Grade
Challenge Pore® PES Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PES Low Protein Binding Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES Low Protein Binding Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PP High-Performance Clarification Capsules & Cartridges

Challenge Pore® PP High-Performance Clarification Capsules & Cartridges
Challenge Filair® PTFE Hydrophobic Sterile Filter with High Retention and High Throughput

Challenge Filair® PTFE Hydrophobic Sterile Filter with High Retention and High Throughput
Challenge Pore® PES Hydrophilic Filter with High Mycoplasma Retention

Challenge Pore® PES Hydrophilic Filter with High Mycoplasma Retention
Challenge Pore® PES Low-Protein-Binding Hydrophilic Sterile Filter

Challenge Pore® PES Low-Protein-Binding Hydrophilic Sterile Filter
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High-Quality Challenge HF® Hollow Fiber Cartridges from China Suppliers - Leading Factory for Biopharmaceutical Purification

In the competitive landscape of biopharmaceuticals, every milligram of your target molecule matters. Our factory in China specializes in providing top-notch separation and purification solutions tailored to enhance purity, maximize recovery, and improve operational efficiency. As leading suppliers in the industry, we are committed to accelerating your journey from research and development to commercial manufacturing, ensuring that your processes are not only efficient but also aligned with the highest standards of quality
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    Product Detail

    Applications
    Harvest, Clarification, Separation and Purification, Concentration, Diafiltration, Buffer Exchange, Microfiltration Trials.
    Products
    Antibody, Vaccines, Gene Therapeutics, Blood Products, Diagnostic Reagents (IVD), Antibiotics, Biochemicals, Natural Products (TCM), Exosomes, Synthetic Biology, Stem Cells.
    Application Cases
    Cell/Microorganism Retention and Perfusion Culture
    High-Density Suspension Culture of Mammalian Cells
    Clarification (Replacing Centrifugation and Depth Filtration)
    Viral Vector Purification
    Concentration and Buffer Exchange of Viral Vectors
    Protein/Antibody Concentration and Diafiltration
    Buffer Exchange
    Separation and Purification of Enzymes and Nucleic Acids
    Desalting, Purification, and Concentration of Various Macromolecular Biologics
    Membrane Material

    The Modified Polyethersulfone (mPES) hollow fiber membranes provided by Challenge IM achieve comprehensive performance enhancements through proprietary modification technology: faster hydrophilicity, higher flux, extremely low non-specific protein adsorption, excellent mechanical strength and chemical stability, and very low leachables levels, providing assurance for your product safety and compliance.

    MWCO (Pore Size or MWCO)

    Classified into two main categories based on pore size: Ultrafiltration membranes standardized by Molecular Weight Cut-Off (MWCO, 1-750KD), and Microfiltration membranes standardized by pore size (0.1-1μm).

    Permeation: The pore size or MWCO should be at least 5-10 times larger than the target molecule.

    Retention: The pore size or MWCO should be 1/3 to 1/5 of the target molecule's molecular weight.

    Fiber Inner Diameter

    Challenge IM offers hollow fiber cartridges with standard fiber inner diameters of 0.5mm and 1.0mm. Reducing the fiber ID increases the membrane packing density per unit filter, thereby increasing membrane surface area and throughput, but it may increase shear effects during fluid circulation.

    1.0mm filters: Mostly used for processing materials with high cell density, high solid content, or high viscosity.

    0.5mm filters: Can be used for most application scenarios to improve mass transfer efficiency.

    Effective Length

    The effective length of the fibers inside the hollow fiber cartridges determine the flow path length, which is related to parameters such as pressure drop across the filter (influencing the filtration driving force).

    The effective length must remain consistent for direct scale-up. Changing the length will significantly alter pressure drop and internal flow fields, preventing linear scale-up. Generally, when processing highly fouling or high-viscosity fluids, it is recommended to choose filters with a shorter effective length.

    Shear Force

    The circulation flow rate of hollow fiber cartridges need to be set by considering the shear tolerance of the process fluid. It is typically 2000-10000 s⁻¹, which is far less than the shear force generated by turbulence on the screen surface of membrane cassettes.

    For general fluids: recommended control at 4000-6000 s⁻¹.

    Shear-sensitive fluids: (e.g., Lentivirus, large proteins expressed by animal cells, LNPs, etc.) Recommended to reduce to approximately 2000 s⁻¹.

    Shear-tolerant fluids: (e.g., small proteins expressed by bacteria) Can be increased to 8000-10000 s⁻¹.

    Frequently Asked Questions
    Q1: What are the key advantages of Challenge IM's Modified Polyethersulfone (mPES) membranes?
    Challenge IM's mPES hollow fiber membranes achieve comprehensive performance enhancements through proprietary modification technology, offering faster hydrophilicity, higher flux, extremely low non-specific protein adsorption, excellent mechanical strength, chemical stability, and very low leachables levels.
    Q2: How should I select the MWCO for target molecule retention and permeation?
    For permeation, the pore size or MWCO should be at least 5-10 times larger than the target molecule. For retention, the pore size or MWCO should be 1/3 to 1/5 of the target molecule's molecular weight.
    Q3: When should I use a 1.0mm fiber inner diameter instead of a 0.5mm fiber?
    1.0mm filters are mostly used for processing materials with high cell density, high solid content, or high viscosity. In contrast, 0.5mm filters can be used for most other application scenarios to improve mass transfer efficiency.
    Q4: Why is maintaining a consistent effective length important for scale-up?
    The effective length must remain consistent for direct scale-up because changing the length significantly alters the pressure drop and internal flow fields, which prevents linear scale-up.
    Q5: What is the recommended shear force limit for shear-sensitive fluids?
    For shear-sensitive fluids (such as Lentivirus, large proteins expressed by animal cells, and LNPs), it is recommended to reduce the shear force to approximately 2000 s⁻¹ to prevent damage.