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Challenge HF® Hollow Fiber Cartridges

Challenge HF® Hollow Fiber Cartridges
Challenge Filair® PTFE High-Retention, High-Flux Hydrophobic Sterilizing-Grade Capsule Filters & Cartridges

Challenge Filair® PTFE High-Retention, High-Flux Hydrophobic Sterilizing-Grade Capsule Filters & Cartridges
Challenge Pore® PES High Mycoplasma Retention Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES High Mycoplasma Retention Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PES Low Protein Binding Hydrophilic Sterilizing-Grade

Challenge Pore® PES Low Protein Binding Hydrophilic Sterilizing-Grade
Challenge Pore® PES Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PES Low Protein Binding Hydrophilic Capsule Filters & Cartridges

Challenge Pore® PES Low Protein Binding Hydrophilic Capsule Filters & Cartridges
Challenge Pore® PP High-Performance Clarification Capsules & Cartridges

Challenge Pore® PP High-Performance Clarification Capsules & Cartridges
Challenge Filair® PTFE Hydrophobic Sterile Filter with High Retention and High Throughput

Challenge Filair® PTFE Hydrophobic Sterile Filter with High Retention and High Throughput
Challenge Pore® PES Hydrophilic Filter with High Mycoplasma Retention

Challenge Pore® PES Hydrophilic Filter with High Mycoplasma Retention
Challenge Pore® PES Low-Protein-Binding Hydrophilic Sterile Filter

Challenge Pore® PES Low-Protein-Binding Hydrophilic Sterile Filter
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China Suppliers of Challenge HF® Hollow Fiber Cartridges for Superior Biopharmaceutical Separation and Purification Solutions

In the highly competitive biopharmaceutical sector, every milligram of your target molecule counts. As leading suppliers in China, we specialize in delivering exceptional separation and purification solutions that guarantee product purity, maximize yield, and enhance operational efficiency. Our advanced technologies accelerate your progress from research and development to commercial manufacturing, enabling your factory to thrive in the precision-driven market. Trust us to support your biopharmaceutical journey with innovative solutions tailored to meet your specific needs
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    Product Detail

    Applications
    Harvest
    Clarification
    Separation and Purification
    Concentration
    Diafiltration
    Buffer Exchange
    Microfiltration Trials
    Products
    Antibody
    Vaccines
    Gene Therapeutics
    Blood Products
    Diagnostic Reagents (IVD)
    Antibiotics
    Biochemicals
    Natural Products (TCM)
    Exosomes
    Synthetic Biology
    Stem Cells
    Application Cases
    ● Cell/Microorganism Retention and Perfusion Culture
    ● High-Density Suspension Culture of Mammalian Cells
    ● Clarification (Replacing Centrifugation and Depth Filtration)
    ● Viral Vector Purification
    ● Concentration and Buffer Exchange of Viral Vectors
    ● Protein/Antibody Concentration and Diafiltration
    ● Buffer Exchange
    ● Separation and Purification of Enzymes and Nucleic Acids
    ● Desalting, Purification, and Concentration of Various Macromolecular Biologics
    Membrane Material

    The Modified Polyethersulfone (mPES) hollow fiber membranes provided by Challenge IM achieve comprehensive performance enhancements through proprietary modification technology: faster hydrophilicity, higher flux, extremely low non-specific protein adsorption, excellent mechanical strength and chemical stability, and very low leachables levels, providing assurance for your product safety and compliance.

    2. MWCO (Pore Size or MWCO)
    Classified into two main categories based on pore size: Ultrafiltration membranes standardized by Molecular Weight Cut-Off (MWCO, 1-750KD), and Microfiltration membranes standardized by pore size (0.1-1μm).

    Permeation

    The pore size or MWCO should be at least 5-10 times larger than the target molecule.

    Retention

    The pore size or MWCO should be 1/3 to 1/5 of the target molecule's molecular weight.

    Fiber Inner Diameter

    Challenge IM offers hollow fiber cartridges with standard fiber inner diameters of 0.5mm and 1.0mm. Reducing the fiber ID increases the membrane packing density per unit filter, thereby increasing membrane surface area and throughput, but it may increase shear effects during fluid circulation.

    1.0mm Filters

    Mostly used for processing materials with high cell density, high solid content, or high viscosity.

    0.5mm Filters

    Can be used for most application scenarios to improve mass transfer efficiency.

    Effective Length

    The effective length of the fibers inside the hollow fiber cartridges determine the flow path length, which is related to parameters such as pressure drop across the filter (influencing the filtration driving force).

    The effective length must remain consistent for direct scale-up. Changing the length will significantly alter pressure drop and internal flow fields, preventing linear scale-up. Generally, when processing highly fouling or high-viscosity fluids, it is recommended to choose filters with a shorter effective length.

    Shear Force

    The circulation flow rate of hollow fiber cartridges need to be set by considering the shear tolerance of the process fluid. It is typically 2000-10000 s⁻¹, which is far less than the shear force generated by turbulence on the screen surface of membrane cassettes.

    General: Recommended control at 4000-6000 s⁻¹.
    Sensitive: (e.g., Lentivirus, large proteins, LNPs) Recommended to reduce to ~2000 s⁻¹.
    Tolerant: (e.g., small proteins expressed by bacteria) Can be increased to 8000-10000 s⁻¹.
    Frequently Asked Questions
    Q What are the key advantages of Challenge IM's Modified Polyethersulfone (mPES) membranes?
    Through proprietary modification technology, our mPES hollow fiber membranes achieve faster hydrophilicity, higher flux, extremely low non-specific protein adsorption, excellent mechanical strength, chemical stability, and very low leachables levels to ensure safety and compliance.
    Q How should I select the MWCO or pore size for filtration?
    For permeation applications, the membrane pore size or MWCO should be at least 5-10 times larger than the target molecule. For retention applications, it should be 1/3 to 1/5 of the target molecule's molecular weight.
    Q When should I choose a 1.0mm fiber inner diameter over a 0.5mm one?
    1.0mm filters are recommended for processing materials with high cell density, high solid content, or high viscosity. 0.5mm filters can be used for most other scenarios to improve mass transfer efficiency.
    Q Why must the effective length remain consistent during scaling up?
    The effective length determines the flow path length and pressure drop. Changing the length alters the internal flow fields and prevents linear scale-up. Shorter lengths are recommended for highly fouling or high-viscosity fluids.
    Q What circulation flow rate/shear force control is recommended for shear-sensitive fluids?
    For shear-sensitive fluids (such as Lentivirus, large proteins, or LNPs), the circulation flow rate should be reduced to approximately 2000 s⁻¹ to minimize damage.